Additional Selective and Differential Cell Culture Media
NEOGEN Universal Beer Agar, 10 kg
Universal Beer Agar is used for the cultivation of bacteria and yeasts encountered in the brewing industry in a laboratory setting. Universal Beer Agar is not intended for use in the diagnosis of disease or other conditions in humans. Universal Beer Agar was developed by Kozulis and Page and used as a basal medium supplemented with beer. The developers compared this formula with other media commonly used in breweries for detecting microbial contamination. The characteristics of Universal Beer Agar are closer to natural environmental conditions found in a typical brewery. Universal Beer Agar supports the growth of more lactic acid bacteria and yields larger colonies in a shorter time than traditional brewer’s media. Supplementing this medium with beer has produced a selective environment for organisms that are adapted to existent conditions in the brewery.For more information visit https://www.neogen.com/solutions/Microbiology/universal-beer-agar
NEOGEN Alkaline Saline Peptone Water (ASPW) (ISO), 500 g
Alkaline Saline Peptone Water is a medium for the enrichment of Vibrio spp. from food and water samples according to ISO 21872-1:2017 and ISO 21872-2:2007, and is not intended for use in the diagnosis of disease or other conditions in humans.Originally described by Shread, Donovan & Lee as an enrichment broth for Aeromonas spp. and identified by Cruickshank as an effective medium for the enrichment of Vibrio spp., Alkaline Saline Peptone Water uses elevated pH and salt levels to provide a favorable environment for the enrichment of Vibrio spp.Peptone provides essential vitamins, minerals, amino acids & nitrogen for growth requirements. Sodium chloride provides essential electrolytes for maintenance of the osmotic balance.Sub-culture onto Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar (NCM0052) will be required.For more information visit https://www.neogen.com/solutions/Microbiology/alkaline-saline-peptone-water-iso
NEOGEN Yersinia Slective Agar (Schiemann's CIN Agar), 500 g
Yersinia Selective Agar is used with cefsulodin and novobiocin for the isolation of Yersinia enterocolitica in a laboratory setting. Yersinia Selective Agar is not intended for use in the diagnosis of disease or other conditions in humans. This medium is based on the work of Schiemann. It is used for the isolation and enumeration of Yersinia spp. from clinical samples and from food. The selective components are sodium desoxycholate, crystal violet, cefsulodin, irgasan and novobiocin. Yersiniae ferment mannitol with an intense, localized, acid production in the center of the colony which produces a red ‘bull’s eye’ appearance. The ratio of transparent border to red centre varies with serotype and environmental strains may appear rough with an irregular edge. Most other enteric bacteria, if they grow, produce a larger colony with a diffuse pinkish center and opaque outer zone.For more information visit https://www.neogen.com/solutions/Microbiology/yersinia-selective-agar
NEOGEN MRS Agar, 500 g
MRS Agar is a medium for the enumeration of mesophilic lactic acid bacteria according to ISO 15214:1998, and is not intended for use in the diagnosis of disease or other conditions in humans. MRS Agar was originally developed in 1960 by de Man, Rogosa & Sharpe for the cultivation and enumeration of Lactobacillus spp. from various sources and is intended as a substitute for Tomato Juice Agar. This original formulation has been adapted and adjusted to pH 5.7 according to ISO 15214:1998. Nutrition is provided by enzymatic digest of casein, glucose, meat & yeast extracts whilst polyoxyethylenesorbitan monooleate, magnesium and manganese sulphates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli whilst inhibiting a number of other organism groups.For more information visit https://www.neogen.com/solutions/Microbiology/mrs-agar
NEOGEN Modified Giolitti & Cantoni Broth, 500 g
Modified Giolitti and Cantoni Broth (ISO) is used for the detection and enumeration of coagulase- positive staphylococci from food and animal feeding stuffs using the Most Probable Number (MPN) technique according to ISO 6888-3:2003. This medium is not intended for use in the diagnosis of disease or other conditions in humans.Originally described by Giolitti and Cantoni as a medium for the enrichment of staphylococci from foodstuffs, Mossel later applied the medium to use with samples from dried milk and infant food. Optimized for use in samples where staphylococci may be stressed and/or in low numbers, growth of the target organisms is promoted by sodium pyruvate, Glycine and the high concentration of mannitol.For more information visit https://www.neogen.com/solutions/Microbiology/modified-giolitti-and-cantoni-broth
NEOGEN Yeast Extract Dextrose Chloramphenicol Agar, 500 g
Yeast Extract Dextrose Chloramphenicol Agar is a selective medium for the enumeration of yeasts and molds in milk and other dairy products, and is not intended for use in the diagnosis of disease or other conditions in humans. The medium is said to have superior storage properties to O.G.Y.E. and also has the advantage of incorporating an autoclavable supplement.For more information visit https://www.neogen.com/solutions/Microbiology/yeast-extract-dextrose-chloramphenicol-agar
NEOGEN Modified Giolitti & Cantoni Broth, 10 kg
Modified Giolitti and Cantoni Broth (ISO) is used for the detection and enumeration of coagulase- positive staphylococci from food and animal feeding stuffs using the Most Probable Number (MPN) technique according to ISO 6888-3:2003. This medium is not intended for use in the diagnosis of disease or other conditions in humans.Originally described by Giolitti and Cantoni as a medium for the enrichment of staphylococci from foodstuffs, Mossel later applied the medium to use with samples from dried milk and infant food. Optimized for use in samples where staphylococci may be stressed and/or in low numbers, growth of the target organisms is promoted by sodium pyruvate, Glycine and the high concentration of mannitol.For more information visit https://www.neogen.com/solutions/Microbiology/modified-giolitti-and-cantoni-broth
NEOGEN Nutrient Broth No. 2, 500 g
Nutrient Both No. 2 is used for the cultivation of fastidious bacteria, and forms the base for Preston Broth according to ISO 10272-1:2017 for the cultivation of Campylobacter spp. This media is not intended for use in the diagnosis of disease or other conditions in humans. This broth can also be used as the suspending medium for cooked meat granules for the cultivation of anaerobic organisms.For more information visit https://www.neogen.com/solutions/Microbiology/nutrient-broth-number-2
NEOGEN Slanetz and Bartley Agar, 500 g
Slanetz and Bartley Medium is used for the enumeration of enterococci from water samples as described in ISO 7899-2, and is not intended for use in the diagnosis of disease or other conditions in humans. This medium was originally described by Slanetz and Bartley for the enumeration of enterococci from water samples using a membrane filtration technique, but it may also be used as a spread plate for the examination of other sample types. Enterococci reduce tetrazolium chloride to the insoluble red dye formazan, producing colonies which are dark red or maroon on the surface of the membrane or agar. This reaction is not exclusive to enterococci, and the count at this stage should be considered presumptive. Colonies may be confirmed as enterococci by demonstrating aesculin hydrolysis using Kanamycin Aesculin Azide Agar (NCM0198).For more information visit https://www.neogen.com/solutions/Microbiology/slanetz-bartley-medium
NEOGEN MacConkey Broth Purple, 500 g
MacConkey Broth Purple is used for the detection and enumeration of fecal coliforms (37°C) and E. coli (44°C). This medium is not intended for use in the diagnosis of disease or other conditions in humans.The replacement of neutral red used in the original formulation by bromocresol purple makes the color change caused by acid producing organisms easier to read.For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-broth-purple
NEOGEN Slanetz and Bartley Agar, 5 kg
Slanetz and Bartley Medium is used for the enumeration of enterococci from water samples as described in ISO 7899-2, and is not intended for use in the diagnosis of disease or other conditions in humans. This medium was originally described by Slanetz and Bartley for the enumeration of enterococci from water samples using a membrane filtration technique, but it may also be used as a spread plate for the examination of other sample types. Enterococci reduce tetrazolium chloride to the insoluble red dye formazan, producing colonies which are dark red or maroon on the surface of the membrane or agar. This reaction is not exclusive to enterococci, and the count at this stage should be considered presumptive. Colonies may be confirmed as enterococci by demonstrating aesculin hydrolysis using Kanamycin Aesculin Azide Agar (NCM0198).For more information visit https://www.neogen.com/solutions/Microbiology/slanetz-bartley-medium
NEOGEN Fastidious Anaerobe Broth, 500 g
Fastidious Anaerobe Agar is used for the cultivation of anaerobic microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans.A primary isolation medium capable of growing most clinically significant anaerobes. Developed by Lab M (Neogen Corporation), comparisons have shown this medium to be superior to other formulations as a primary isolation medium for fastidious organisms. The peptones included have been chosen for maximum growth stimulation. Starch and sodium bicarbonate act as de-toxification agents while hemin encourages pigment production in Porphyromonas melaninogenicus. Specific growth promoting agents are Cysteine for Fusobacterium necrophorum, Propionibacterium acne and Bacteriodes fragilis, arginine for Eubacterium spp. soluble pyrophosphate for Porph. gingivalis and Porph. asaccharolyticus.For more information visit https://www.neogen.com/solutions/Microbiology/fastidious-anaerobe-broth
NEOGEN Kanamycin Aesculin Azide Agar, 500 g
Kanamycin Aesculin Azide Agar is a selective isolation and enumeration medium for enterococci (Lancefield group D streptococci) in food, and is not intended for use in the diagnosis of disease or other conditions in humans.Sodium azide and kanamycin provide the selective inhibition required whilst aesculin and iron salts form an indicator system for the presumptive identification of enterococci. Incubation at 42°C will increase the medium’s selectivity.Formula:Tryptone - 20.0 g/LYeast Extract - 5.0 g/LSodium Chloride - 5.0 g/LSodium Citrate - 1.0 g/LAesculin - 1.0 g/LFerric Ammonium Citrate - 0.5 g/LSodium Azide - 0.15 g/LKanamycin Sulphate - 0.02 g/LAgar- 10.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/kanamycin-aesculin-azide-agar
NEOGEN LESS Plus Medium Irradiated, 500 g
Listeria monocytogenes, described first in 1926 by Murray, Webb and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis indicates the principle route of transmission is via the consumption of foods contaminated with Listeria monocytogenes. Implicated vehicles of transmission include meat, eggs, chicken, vegetables, and dairy products. Listeria spp. are ubiquitous in nature, present in a wide range of unprocessed foods and in soil, sewage, and river water. Certain strains of Listeria spp. are able to survive the manufacturing and ripening processes in dairy products.For more information visit https://www.neogen.com/solutions/Microbiology/less-plus-medium
NEOGEN Baird Parker Agar Base, 500 g
Baird Parker Agar is used for the cultivation of Staphylococcus aureus from foodstuffs according to ISO 6888, and is not intended for use in the diagnosis of disease or other conditions in humans.Originally introduced in 1962, this medium was developed by Baird-Parker to overcome the problems of recovering damaged Staphylococcus aureus from foodstuffs. This version of the medium is formulated according to ISO 6888-1:1999+A1:2003 and is in compliance with ISO 6888-2:2003+A1:2003 and ISO 6888-3:2003.Baird-Parker medium is highly selective by nature, due to the presence of potassium tellurite and lithium chloride. Tellurite inhibits most coliforms and is also reduced to telluride by S. aureus, giving the typical black colonies. Glycine and sodium pyruvate are both used as growth factors by staphylococci while the pyruvate also neutralizes any toxic peroxides that may be formed.For more information visit https://www.neogen.com/solutions/Microbiology/baird-parker-agar-base